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. Author manuscript; available in PMC: 2018 Jan 12.
Published in final edited form as: Cell. 2016 Nov 17;168(1-2):20–36. doi: 10.1016/j.cell.2016.10.044

Table 1.

Properties of some of the naturally occurring and engineered CRISPR enzymes that have been used for genome editing in mammalian cells.

Enzyme name Size (residues) PAM requirement and cleavage pattern
SpCas9/FnCas9 1368/1629 graphic file with name nihms826123t1.jpg
St1Cas9 1121 graphic file with name nihms826123t2.jpg
St3Cas9 1409 graphic file with name nihms826123t3.jpg
NmCas9 1082 graphic file with name nihms826123t4.jpg
SaCas9 1053 graphic file with name nihms826123t5.jpg
AsCpf1/LbCpf1 1307/1228 graphic file with name nihms826123t6.jpg
VQR SpCas9 1368 graphic file with name nihms826123t7.jpg
EQR SpCas9 1368 graphic file with name nihms826123t8.jpg
VRER SpCas9 1368 graphic file with name nihms826123t9.jpg
RHA FnCas9 1629 graphic file with name nihms826123t10.jpg
KKH SaCas9 1053 graphic file with name nihms826123t11.jpg
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