Table 1.
Properties of some of the naturally occurring and engineered CRISPR enzymes that have been used for genome editing in mammalian cells.
Enzyme name | Size (residues) | PAM requirement and cleavage pattern |
---|---|---|
SpCas9/FnCas9 | 1368/1629 |
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St1Cas9 | 1121 |
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St3Cas9 | 1409 |
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NmCas9 | 1082 |
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SaCas9 | 1053 |
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AsCpf1/LbCpf1 | 1307/1228 |
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VQR SpCas9 | 1368 |
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EQR SpCas9 | 1368 |
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VRER SpCas9 | 1368 |
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RHA FnCas9 | 1629 |
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KKH SaCas9 | 1053 |
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