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. Author manuscript; available in PMC: 2018 Jan 15.

Published in final edited form as: Acta Biomater. 2016 Oct 12;48:144–156. doi: 10.1016/j.actbio.2016.10.016

Peroxiredoxin 2 (Prx 2) antioxidant enzyme staining of rat dermal fibroblasts (A, C, and E) and rat tendon fibroblasts (B, D, and F) exposed directly to 5 min- or 16 hrs-cured hydrogels for 2 hours. Cell nuclei and Prx 2 were stained by DAPI (blue) and anti-Prx2 antibody (green), respectively. Fluorescent intensity of Prx 2 per cell in rat dermal and tendon fibroblasts (G). ^$ p < 0.05 when compared to both the media control and the 16 hrs-cured hydrogel groups. * p < 0.05 when compared to the media control. Scale bar: 100 μm.

Peroxiredoxin 2 (Prx 2) antioxidant enzyme staining of rat dermal fibroblasts (A, C, and E) and rat tendon fibroblasts (B, D, and F) exposed directly to 5 min- or 16 hrs-cured hydrogels for 2 hours. Cell nuclei and Prx 2 were stained by DAPI (blue) and anti-Prx2 antibody (green), respectively. Fluorescent intensity of Prx 2 per cell in rat dermal and tendon fibroblasts (G). ^$ p < 0.05 when compared to both the media control and the 16 hrs-cured hydrogel groups. * p < 0.05 when compared to the media control. Scale bar: 100 μm.