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. Author manuscript; available in PMC: 2018 Jan 15.

Published in final edited form as: Acta Biomater. 2016 Oct 12;48:144–156. doi: 10.1016/j.actbio.2016.10.016

Immunofluorescent staining of macrophages (CD 68) in tissue surrounding 5 min- and 16 hrs-cured PEG-D4 hydrogels after 3 (A, B) and 7 (C, D) days of subcutaneous implantation. Cell nuclei and macrophages were stained by DAPI (blue) and CD68 (red), respectively. The letter h indicates the location of the implanted hydrogel. The percentage of macrophages relative to the total number of cells stained with DAPI (E). * p < 0.05 when compared to 5 min-cured hydrogels after 3 days of subcutaneous implantation; ^$ p < 0.05 when compared to 5 min-cured hydrogels after 7 days of subcutaneous implantation; ^& p < 0.05 when compared to 16 hrs-cured hydrogels after 3 days of subcutaneous implantation. Scale bar: 100 μm.

Immunofluorescent staining of macrophages (CD 68) in tissue surrounding 5 min- and 16 hrs-cured PEG-D4 hydrogels after 3 (A, B) and 7 (C, D) days of subcutaneous implantation. Cell nuclei and macrophages were stained by DAPI (blue) and CD68 (red), respectively. The letter h indicates the location of the implanted hydrogel. The percentage of macrophages relative to the total number of cells stained with DAPI (E). * p < 0.05 when compared to 5 min-cured hydrogels after 3 days of subcutaneous implantation; ^$ p < 0.05 when compared to 5 min-cured hydrogels after 7 days of subcutaneous implantation; ^& p < 0.05 when compared to 16 hrs-cured hydrogels after 3 days of subcutaneous implantation. Scale bar: 100 μm.