Body weight gain, composition, and food intake in mice fed low vs. high LA HFD. (A) Three month old male mice fed either CON, 1%, 15% or 22.5% were weighed weekly for 12 weeks. Asterisks at weeks 7 and 8 denote comparison between 1% and 22.5% and at weeks 9–12 between 1% and both 15% and 22.5%. (B) Weekly food weight (g) was summed and multiplied by caloric content (kcals/g) to calculate cumulative intake in kcals. (C) Feeding efficiency was calculated by dividing weight gained (g) by food consumed (kcals) over 12 weeks. (D) Body composition was measured after 12 weeks by EchoMRI; body fat was divided by body weight. Data (n=12) were analyzed by a repeated measures, two-way ANOVA (A) or one-way ANOVA (B, C, D) with Bonferroni-Dunn multiple comparisons test. (* p<0.05; ** p<0.01; **** p<0.0001).