Table 2.
Primer sequences
| Gene Name |
Forward Primer | Reverse Primer | Accession # |
|---|---|---|---|
| Actb | GCCCTGAGGCTCTTTTCCA | TAGTTTCATGGATGCCACAGGA | NM_007393.3 |
| Agrp | CTCCACTGAAGGGCATCAGAA | ATCTAGCACCTCCGCCAAA | NM_007427.2 |
| Cart | GCTCAAGAGTAAACGCATTCC | GTCCCTTCACAAGCACTTCAA | NM_013732 |
|
Cx3cl 1 |
ACGAAATGCGAAATCATGTGC | CTGTGTCGTCTCCAGGACAA | NM_009142.3 |
| Hprt | GCTTGCTGGTGAAAAGGACCTCTCGAA G |
CCCTGAAGTACTCATTATAGTCAAGGGC AT |
NM_013556 |
| Icam1 | GTGATGCTCAGGTATCCATCCA | CACAGTTCTCAAAGCACAGCG | NM_010493.2 |
| Ikk | CCATATCCTGGCTGTCACCT | GGCACCTTGGATGACCTAGA |
NM_001159774. 1 |
| Il-1 | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT | NM_008361.3 |
| Il-6 | GAGATCGACTCTCTGTTCGAGG | GCCCGTTGAAGAAGTCCTG | NM_010479.2 |
| Il-15 | ACATCCATCTCGTGCTACTTGT | GCCTCTGTTTTAGGGAGACCT | NM_008357.2 |
| Insr | GTGTTCGGAACCTGATGAC | GTGATACCAGAGCATAGGAG | NM_010568 |
| Lepr | AGAATGACGCAGGGCTGTAT | TCCTTGTGCCCAGGAACAAT | NM_146146.2 |
| Npy | ACTGACCCTCGCTCTATCTC | TCTCAGGGCTGGATCTCTTG | NM_023456 |
| Pai1 | TTCAGCCCTTGCTTGCCTC | ACACTTTTACTCCGAAGTCGGT | NM_008871.2 |
| Pomc | GGAAGATGCCGAGATTCTGC | TCCGTTGCCAGGAAACAC | NM_008895 |
| Socs3 | TTCACGGCTGCCAACATCT | GCTAGTCCCGAAGCGAAATCT | NM_007707.3 |
| Stat3 | TTCCTGGCACCTTGGATTG | CGAAGGTTGTGCTGATAGAG | NM_213659.2 |
| Tlr4 | ATGGCATGGCTTACACCACC | GAGGCCAATTTTGTCTCCACA | NM_021297.2 |
| Traf6 | ATCTCTGAGGATCATCAAGTACATTGT | TGTGTGTATTAACCTGGCACTTCTG | NM_009424.2 |