Fig. 5.

KDM1A and CoREST associate with oligomeric but not monomeric CtBP. (A) Coimmunoprecipitation of CtBP and KDM1A. Presence of KDM1A in Flag immunoprecipitates of CtBP depleted HuTu 80 cells expressing Flag-CtBP1 proteins (WT^R or DM^R) in nuclear extracts. Precipitated proteins were detected by immunoblotting with the indicated antibody. Flag and KDM1A input shown by immunoblotting (lower panels). * denotes a nonspecific band. (B) Association of RREB1 and CoREST with oligomeric and monomeric forms of CtBP. Flag immunoprecipitates were examined for the expression of RREB1 and CoREST by immunoblotting.