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Injection sies
| A/P (Lambda) | M/L (Bregma - Lambda) | D/V (skull) | Volume |
|---|---|---|---|
| +0.4 | ±3.8 | −6.5/−6.2 | 0.2–0.4 μL |
| −0.1 | ±3.7 | −6.0/−5.7 | 0.2–0.4 μL |
| −0.6 | ±3.4 | −4.6/−4.3 | 0.2–0.4 μL |
| −1.0 | ±3.1 | −4.5/−4.0 | 0.2–0.4 μL |
A/P and M/L coordinates are relative to Lambda, and D/V is relative to the skull surface. All injections are administered at a 22° M/L angle.
