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. Author manuscript; available in PMC: 2018 Jan 20.
Published in final edited form as: J Mol Biol. 2016 Dec 1;429(2):177–191. doi: 10.1016/j.jmb.2016.11.024

Figure 2.

Figure 2

Two orientations for reporting PAM sequences. The PAM sequence is located within double-stranded DNA, where either strand of the PAM can be reported along with its location relative to the protospacer. Under the target-centric orientation, the PAM is reported from the strand that base pairs with the guide RNA. Under the guide-centric orientation, the PAM is reported from the strand that matches the guide RNA and is used for guide-RNA design.