Table 4.

Plasma amino acid concentrations (µM) in fasted and Leu gavaged mice on high fat diet (HFD)

	WT -HFD	LivTg-HE-HFD
Leu	897.6 ± 230	449.8 ± 302*
Ile	68.4 ± 12	55.8 ± 6
Val	167.1 ± 20	157.5 ± 15
His	25.1 ± 1	27.8 ± 3.3
Met	88.9 ± 11	100.7 ± 10
Phe	57.5 ± 3	81.6 ± 6*
Trp	95.4 ± 8.	125.2 ± 5*
Thr	159.1 ± 14	200.9 ± 15
Lys	326.0 ± 37	381.1 ± 14
Glu	108.0 ± 14	126.1 ± 22
Gln	850.1 ± 68	1008.5 ± 79
Asp	48.0 ± 8	43.1 ± 10
Asn	40.3 ± 4	50.2 ± 4
Ala	1198.9 ± 102	1155.8 ± 87
Gly	393.5 ± 39	434.6 ± 21
Ser	161.8 ± 32	187.2 ± 17
Tyr	96.9 ± 12	175.2 ± 19*
Cit	53.8 ± 4	70.9 ± 7

WT-HFD and LivTg HE-HFD male mice were fed HFD for 18 weeks, fasted for 12 h, then gavaged with Leu for 30 min before drawing blood for analysis (see Methods). Data represent mean ± SEM n=6–12 male mice, age 24 weeks,

^*P≤0.05 as compared to WT-HFD mice.