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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Acta Biomater. 2016 Oct 18;48:258–269. doi: 10.1016/j.actbio.2016.10.027

Figure 8. Effect of matrix stiffening on gene expression in pancreatic stellate cells.

Figure 8

mRNA samples were collected from cell-laden hydrogels before stiffening (day-1), as well as day-2 and day-6 post-stiffening using 1 kU/mL tyrosinase (i.e., day-3, day-7 culture time). Soft: Cell-laden hydrogels crosslinked by regular MMP-sensitive peptide (i.e., 0Y peptide). Stiffened: Cell-laden hydrogels crosslinked by 2Y peptide. GAPDH was used as the housekeeping gene and the expression levels of αSMA (A), CTGF (B), and HIF1α (C) in different samples were normalized to that in day-1 soft gel (1-fold). Experiments were repeated independently for three times with three samples in each group. (Data represent Mean ± SEM). **p<0.01, ***p<0.001.