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. 2017 Jan 14;23(2):204–215. doi: 10.3748/wjg.v23.i2.204

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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LiverChip^® three-dimensional perfused cell culture system. A: The LiverChip^® plate contains 12 independent bioreactors for the 3D culture of hepatocytes; B: Hepatocytes form 3D micro-tissue structures in an array of channels in a collagen I-coated scaffold that is contained in each LiverChip^® well; C: Schematic representation of how media flows through the channels of each LiverChip scaffold due to the action of a pneumatically operated pumps in the base of the plate. The speed and direction of flow can be adjusted using an electronic controller. 3D: Three-dimensional.