FIG 7.
Oligomeric state of ClpP1 and ClpP2. (A) Analytical gel filtration of ClpP1 and ClpP2 at 10 μM load concentration showed tetradecamer (expected molecular mass, ∼323 kDa) and heptamer (expected molecular mass, ∼163 kDa), respectively. Size standard elution volumes are marked with arrows and values given in kilodaltons. (B) The distribution of ClpP2 by SV-AUC [c(s20,w)] is consistent with heptamer. Centrifugation was performed with 1 mg/ml protein in 0.1 M Tris (pH 8.5) and 0.15 M NaCl at 20°C. SEC-MALS data shown in Fig. S7 were also consistent with ClpP2 being a stable heptamer.