Motility in A. baumannii is controlled by OmpR/EnvZ. Cultures were grown in LB medium (−NaCl) to an OD600 of 0.3, and a 1-μl drop was then applied to the surface of 0.3% Eiken agar (−NaCl) plates, after which the plates were incubated at 37°C for ∼9 h. Bars represent the average diameters of migration, with error bars representing the standard deviations. (A) Motility quantifications for opaque variants of the wild-type strain AB5075, the ΔompR mutant (KT4), the ΔenvZ mutant (KT5), and the ΔompR ΔenvZ double mutant (KT6). (B) Motility quantifications for translucent variants of the wild-type strain AB5075, the ΔompR mutant (KT4), the ΔenvZ mutant (KT5), and the ΔompR ΔenvZ double mutant (KT6). ***, P < 0.0001; **, P < 0.005; *, P < 0.05.