Fig. 3.

Keratinocyte migration after exposure to healthy and sepsis sera (horizontal wound-healing assay). a An example of the wound surface area calculation from the microscopy images of cell migration in wounds after 0, 24 and 48 h exposure to healthy or sepsis sera. White dotted lines mark the open wound area. The open surface area was measured using ImageJ. b Closure of open wound areas of keratinocytes at 0, 24 and 48 h (% of 0 h area). The data present means with standard deviations from eight scratch wounds incubated with day 1, 4, and 9 sepsis sera and means with standard deviations from four healthy sera control wounds