Table 1.
The panel of targeted genes involved in mTOR pathway
| Gene | Chr | Number of exons | Number of amplicons | Total Bases | Overall coverage (%) |
|---|---|---|---|---|---|
| PIK3CA | 3 | 20 | 34 | 3407 | 98.3 |
| PIK3CB | 3 | 22 | 30 | 3433 | 98.7 |
| PIK3CD | 1 | 22 | 37 | 3355 | 97.6 |
| PIK3R1 | 5 | 18 | 23 | 2506 | 100 |
| PIK3R2 | 19 | 15 | 19 | 2337 | 79.2 |
| PIK3R3 | 1 | 10 | 14 | 1486 | 100 |
| PTEN | 10 | 9 | 12 | 1302 | 97.6 |
| PDPK1 | 16 | 14 | 19 | 1811 | 95.3 |
| AKT1 | 14 | 13 | 22 | 1573 | 97.5 |
| AKT2 | 19 | 14 | 18 | 1687 | 98.9 |
| AKT3 | 1 | 14 | 17 | 1624 | 98.8 |
| RHEB | 7 | 8 | 8 | 635 | 95.1 |
| MTOR | 1 | 57 | 69 | 8220 | 99.7 |
| TSC1 | 9 | 22 | 29 | 3834 | 100 |
| TSC2 | 6 | 42 | 61 | 6006 | 96.5 |
| Total | 300 | 412 | 43216 | 97.4 |
Chr chromosome
Overall coverage means percent of coverage in target sequence