Fig. 3.

Proposed cellular location of I. scapularis proteins investigated in the present study. Expression of FAH, ERP29, ALDH, VNN, MDH2, PARP, CMPK, ACAT1, Hypo195 and Hypo576 (light blue) is shown based on studies of orthologous proteins in H. sapiens (excluding Hypo195 and 576). Black circles, ribosomes; dark blue lines, LGTV positive strand; orange lines, LGTV negative strand; red circles, viral proteins associated with replication complexes on the endoplasmic reticulum; purple particles, immature LGTV prior to cleavage of the pr peptide; magenta circles, furin protease produced by the host cell and responsible for cleavage of the pr peptide from the Membrane protein, producing mature LGTV; extracellular purple circles, cleaved pr peptide; extracellular black smooth particles, mature LGTV. Protein localization was based on COMPARTMENTS [33] predictions using data from H. sapiens