Table 1.
Summary of Ixodes scapularis genes selected for RNAi analyses
| Tick Protein; KEGGa Entry | I. scapularis VectorBase accession ID | GenBank accession number | Predicted function | % amino acid identity to H. sapiens ortholog | Location of gene on IscaW1 (scaffold: bp range) | Number of paralogs identified in IscaW1 assembly | Predicted number of protein binding partnersb |
|---|---|---|---|---|---|---|---|
| Fumarylacetoacetase (FAH) | ISCW020196 | XP_002407463 | Amino acid metabolism | 65.9 | DS831757: 33,311–44,738 | – | 2 |
| Endoplasmic reticulum protein 29 (ERP29) | ISCW018425 | XP_002435676 | Protein folding, sorting, & degradation | 17 | DS758338: 84,585–88,966 | – | 0 |
| Aldehyde dehydrogenase (ALDH) | ISCW015982 | XP_002399265 | Amino acid metabolism | 56.7 | DS612682: 208,981–240,639 | 16 | 6 |
| Carbon-nitrogen hydrolase/pantetheine hydrolase/vanin-like (VNN) | ISCW004822 | XP_002402506 | Metabolism of cofactors & vitamins | 33.9 | DS712062: 22,027–47,303 | – | 0 |
| Malate dehydrogenase (MDH2) | ISCW003528 | XP_002402153 | Carbohydrate metabolism | 68.9 | DS711115: 47,872–64,175 | – | 21 |
| Poly [ADP-ribose] polymerase (PARP) | ISCW019519 | XP_002409668 | DNA replication & repair | 48.6 | DS807313: 23,492–74,749 | 2 | 30 |
| Cytidine/uridine monophosphate kinase (CMPK) | ISCW012446 | XP_002413690 | Nucleotide metabolism | 40.2 | DS915558: 7422–14,364 | 3 | 2 |
| Acetyl-CoA C-acetyltransferase (ACAT1) | ISCW016117 | XP_002402965 | Carbohydrate, lipid, amino acid, terpenoid/polykeytide metabolism | 60.2 | DS624476: 15,968–41,821 | 2 | 24 |
| Hypothetical protein (Hypo195) | ISCW011195 | XP_002411582 | Unknown | – | DS857119: 268,751–271,378 | – | – |
| Hypothetical protein (Hypo576) | ISCW020576 | XP_002408828 | Unknown | – | DS835548: 71,893–118,756 | – | – |
aKyoto Encyclopedia of Genes and Genomes; http://www.genome.jp/kegg/
bNumber of potential protein binding partners as predicted via STRING