Table 2.
Results of RNAi knockdown of I. scapularis transcripts on LGTV infection in ISE6 cells
| Tick Protein | VectorBase accession ID | Effect of treatment on ISE6 protein expressiona | Effect of transcript knockdown on LGTV negative strand replication | Effect of transcript knockdown on release of infectious LGTV | |
|---|---|---|---|---|---|
| LGTVb | UV-LGTVb | ||||
| Fumarylacetoacetase (FAH) | ISCW020196 | Up | Up | No change | Decreased |
| Endoplasmic reticulum protein 29 (ERP29) | ISCW018425 | Up | Up | No change | Decreased |
| Aldehyde dehydrogenase (ALDH) | ISCW015982 | Up | Up | No change | Decreased |
| Carbon-nitrogen hydrolase/pantetheine hydrolase/vanin-like (VNN) | ISCW004822 | Up | NC | Decreased | Decreased |
| Malate dehydrogenase (MDH2) | ISCW003528 | Up | NC | No change | Decreased |
| Poly [ADP-ribose] polymerase (PARP) | ISCW019519 | Up | NC | No change | No change |
| Cytidine/uridine monophosphate kinase (CMPK) | ISCW012446 | Up | Up | No change | Decreased |
| Acetyl-CoA C-acetyltransferase (ACAT1) | ISCW016117 | Up | Up | Decreased | Decreased |
| Hypothetical protein (Hypo195) | ISCW011195 | Up | NC | No change | Decreased |
| Hypothetical protein (Hypo576) | ISCW020576 | Up | Up | Decreased | Decreased |
aProtein expression following LGTV and/or UV-LGTV treatment of ISE6 cells compared to mock treatment [4]. Up = increased expression and NC = no statistically-significant change in expression
bLGTV = LGTV infection; UV-LGTV = UV inactivated LGTV treatment