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. 2017 Jan 13;18:76. doi: 10.1186/s12864-016-3416-5

Table 4.

Classification intervals, giving the range of heterozygote marker counts leading to Fn classification under different assumptions of allele fixedness, and the probability of the classification of the specific back cross generation being correct, using a maximum likelihood model

Diagnostic markers Introgressing MAF = 0.05 MAF = 0.05
from to prob. correct from to prob. correct from to prob. correct
F1 36 50 1.000 36 50 1.000 36 50 1.000
F2 19 35 0.966 18 35 0.963 20 35 0.940
F3 10 18 0.808 9 17 0.836 13 19 0.687
F4 5 9 0.677 5 8 0.590 9 12 0.501
F5 3 4 0.411 3 4 0.402 7 8 0.306
F6 2 2 0.261 2 2 0.254 6 6 0.171
F7 1 1 0.361 1 1 0.357
Native 0 0 1.000 0 0 1.000 0 4 0.479

Note that with minor allele frequency of 0.05, there is no number of heterozygote markers where the F7 generation is most likely to produce, and thus we will never classify a specimen as F7. The same holds for F8 and above under all assumptions of minor allele frequencies.