Table 4.
Classification intervals, giving the range of heterozygote marker counts leading to Fn classification under different assumptions of allele fixedness, and the probability of the classification of the specific back cross generation being correct, using a maximum likelihood model
Diagnostic markers | Introgressing MAF = 0.05 | MAF = 0.05 | |||||||
---|---|---|---|---|---|---|---|---|---|
from | to | prob. correct | from | to | prob. correct | from | to | prob. correct | |
F1 | 36 | 50 | 1.000 | 36 | 50 | 1.000 | 36 | 50 | 1.000 |
F2 | 19 | 35 | 0.966 | 18 | 35 | 0.963 | 20 | 35 | 0.940 |
F3 | 10 | 18 | 0.808 | 9 | 17 | 0.836 | 13 | 19 | 0.687 |
F4 | 5 | 9 | 0.677 | 5 | 8 | 0.590 | 9 | 12 | 0.501 |
F5 | 3 | 4 | 0.411 | 3 | 4 | 0.402 | 7 | 8 | 0.306 |
F6 | 2 | 2 | 0.261 | 2 | 2 | 0.254 | 6 | 6 | 0.171 |
F7 | 1 | 1 | 0.361 | 1 | 1 | 0.357 | |||
Native | 0 | 0 | 1.000 | 0 | 0 | 1.000 | 0 | 4 | 0.479 |
Note that with minor allele frequency of 0.05, there is no number of heterozygote markers where the F7 generation is most likely to produce, and thus we will never classify a specimen as F7. The same holds for F8 and above under all assumptions of minor allele frequencies.