Figure 1. A psoralen-ICL is unhooked by an incision-independent pathway that does not require FANCI-D2.

(A) Cartoons of pICL^Pso and pICL^Pt. NheI and SapI restriction sites coincide with the psoralen-and cisplatin-ICLs, respectively.

(B) Cartoon of pICL^Pt replication intermediates digested with HincII.

(C) pCtrl, pICL^Pt, or pICL^Pso was replicated in egg extracts with [α-³²P]dATP. Replication intermediates were separated on a native agarose gel and visualized by autoradiography. SC, supercoiled. OC, open circular. HR, homologous recombination intermediate, also denoted by white arrowhead.

(D) Cartoon of pICL^Pso replication intermediates digested with HincII.

(E) pICL^Pt or pICL^Pso was replicated in egg extracts with [α-³²P]dATP. Replication intermediates were digested with HincII, separated on a native agarose gel, and visualized by autoradiography. White arrowheads, 3.6 kb and 2.0 kb incision products depicted in (B).

(F) pICL^Pt or pICL^Pso was replicated in mock or FANCI-D2 depleted egg extracts in the presence of [α-³²P]dATP, and the repair intermediates were analyzed as in (C). White arrowhead, Figure 8 DNA structure. The fraction Figure 8 indicates the proportion of Figure 8 structures relative to total species at 300 min.