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. Author manuscript; available in PMC: 2017 Oct 6.
Published in final edited form as: Cell. 2016 Sep 29;167(2):498–511.e14. doi: 10.1016/j.cell.2016.09.008

Figure 3. Psoralen-ICL undergoes FANCI-D2 dependent processing when the N-glycosyl bond cannot be cleaved.

Figure 3

(A) Schematic of a psoralen-ICL formed between two 2′-fluoroarabino-dT (FdT) nucleosides (inset).

(B) pICLPt, pICLPso, or pICLFdT-Pso was replicated in egg extracts in the presence of [α-32P]dATP. Repair intermediates were analyzed as in Figure 1C.

(C) pICLFdT-Pso was replicated in mock-depleted egg extract, FANCI-D2 depleted extract (ΔI-D2), or ΔI-D2 extract supplemented with xFANCI-D2 (ΔI-D2 + xI-D2) in the presence of [α-32P]dATP, and the repair intermediates were analyzed as in Figure 1C. White arrowhead, Figure 8 DNA structure that persists in the absence of FANCI-D2. The fraction Figure 8 indicates the proportion of Figure 8 structures relative to total species at 300 min.