KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-FANCD2	Knipscheer et al., 2009	Rabbit 20019
Rabbit polyclonal anti-FANCI	Duxin et al., 2014	Rabbits 26651 and 26864
Rabbit polyclonal anti-CDC45	Walter and Newport, 2000	Rabbit 534
Rabbit polyclonal anti-MCM6	New England Peptide	Project #2926
Rabbit polyclonal anti-H3	Cell Signaling Technology	Cat #9715
Rabbit polyclonal anti-Rev1	Budzowska et al., 2015	Rabbits 714 and 1010
Rabbit polyclonal anti-NEIL3	New England Peptide; this study	Project #3470
Rabbit polyclonal anti-NEIL3	Abgent; this study	Rabbit 57141
Chemicals, Peptides, and Recombinant Proteins
Recombinant Xenopus laevis NEIL3-FLAG	this study	N/A
Recombinant Xenopus laevis NEIL3-FLAG K60A	this study	N/A
Recombinant Xenopus laevis FANCI-FANCD2	Knipscheer et al., 2009	N/A
2'-fluoroarabino-dT (FdT)	LINK Technologies	N/A
NMS-873	Sigma	Cat #SML1128
Trioxsalen	Sigma	Cat #T6137
Critical Commercial Assays
Thermo Sequenase Cycle Sequencing Kit	USB	Cat#785001KT
Random Primed DNA Labeling Kit	Roche	Cat#11004760001
Bac to Bac Expression System	ThermoFisher	Cat#10359016
Deposited Data
Experimental Models: Cell Lines
Sf9 insect cells	ThermoFisher	Cat#B82501
Experimental Models: Organisms/Strains
Xenopus laevis	Nasco	Cat #LM0053MX
Recombinant DNA
pICL-lacOPt	Zhang et al., 2015	N/A
pICL-lacOPso	Zhang et al., 2015	N/A
pICL-lacOAP	this study	N/A
pICL-lacOAP-reverse	this study	N/A
pCtrl-lacO	Dewar et al., 2015	N/A
pICLPt	Raschle et al., 2008	N/A
pICLAP	this study	N/A
pCtrl	Raschle et al., 2008	N/A
pFastBac1-NEIL3-FLAG	this study	N/A
pFastBac1-NEIL3K60A-FLAG	this study	N/A
Sequence-Based Reagents
See Table S1 for sequences of primers and oligonucleotides used in the preparation of pICLs and AP-ICL unhooking substrates	this study	N/A
Software and Algorithms
ImageJ	Abramoff et al., 2004	N/A
Other