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Sialylated O-glycans of mucin from bovine submaxillary glands (MBS) by MALDI-MS. a The sialic acids that were stabilized by carbodiimide coupling have a significantly increased MS signal; b the sialic acids without modification have low intensity in MALDI-MS. An internal standard (Neurotensin, 20 μM/1 μL) was spiked in the sample. The sialic acid modified glycans have one sodium adduct [Na]+, while native glycans have an extra sodium adduct per sialic acid
