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. 2017 Jan 13;18:83. doi: 10.1186/s12864-016-3455-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Combining dry and wet bench processes to identify functional enolases in the silkworm B. mori. To identify enolase sequences in B. mori, we performed a HMM search of public databases. We found five enolase sequences, which we then characterized using RNA-seq analysis, cDNA cloning, and RT-PCR. Finally, we determined that three enolase genes in B. mori were functional. The insect experimental tools and machines drawings (http://togotv.dbcls.jp/ja/pics.html ) are licensed at (http://creativecommons.org/licenses/by/4.0/deed.en)