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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Nov 30;1859(2):289–293. doi: 10.1016/j.bbamem.2016.11.013

Figure 1. CFTR requires phosphatidylserine for maximum ATPase activity.

Figure 1

Aliquots of wild-type CFTR were preincubated on ice with the indicated lipids for 15 min prior to substrate addition, which was 1/5th of the final assay volume. Extent of ATP hydrolysis was then determined. Final lipid concentrations in assay are given. Representative titration data are shown, and have been duplicated for each lipid with similar results. The data are displayed in three panels for clarity.

Brain polar lipid extract contained 33.1% PE, 18.5% PS, 12.6% PC, 4.1% PI, 0.8% phosphatidic acid, and 30.9% unknown, w/w.