Table 1.
Phosphatidylserine improves thermal stability of wild-type CFTR ATPase
| functional Tm, °C | Tm shift, °C | P valuea | |
|---|---|---|---|
| no addition | 22.7 ± 0.8 (7) | ||
| PE/PS/PC/cholesterol 5:3:1:1 | 31.5 ± 1.1 (3) | ±8.8 | <0.0001 |
| PE/PS/PC/cholesterol 5:0:1:1 | 24.9 ± 0.6 (3) | ±2.2 | 0.003 |
| cholesterol | 23.0 ± 0.7 (3) | ns | |
| liver PI | 22.7 ± 1.4 (3) | ns | |
| POPE | 26.0 ± 1.1 (3) | ±3.3 | 0.0006 |
| POPC | 23.9 ± 0.6 (3) | ns | |
| POPS | 32.6 ± 0.9 (4) | ±9.9 | <0.0001 |
| porcine brain PS | 35.0 ± 0.2 (3) | ±12.3 | <0.0001 |
| porcine brain sphingomyelin | 21.9 ± 1.6 (3) | ns | |
| 0.3 mM glibenclamide | 22.6 ± 0.9 (3) | ns | |
| 1.5% dimethylsulfoxideb | 22.7 ± 0.4 (3) | ns |
Functional Tms were derived from thermal inactivation data as illustrated in Figure 2. Data represent mean ± standard deviation, with number of replicate experiments given in parentheses.
a
two-tailed Student’s t-test for difference from the no lipid control.
ns: not statistically significant (P >0.05)
b
vehicle control for glibenclamide