Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Kidney Int. 2016 Dec 15;91(2):501–511. doi: 10.1016/j.kint.2016.09.044

Figure 1. Number of Glomerular Sections Required for Protein Identification.

Figure 1

Glomerular sections were retrieved from 10 µm human kidney tissue sections of frozen (FR) and formalin-fixed paraffin embedded (FFPE) and extracted using 4% SDS prior to LCMS analysis. To establish optimal numbers of glomerular sections (x-axis) for LCMS analysis, numbers of MS2 events (left y-axis) and identified proteins (right y-axis) were plotted against the number of glomerular sections. 1a. Total number of MS2 events and proteins identified from varying numbers of glomerular sections in FR tissue. 1b. Total number of MS2 events and proteins identified from varying numbers of glomerular sections in FFPE tissue.