The efficiency of cellular and extracellular proteome isolation was examined by comparing the distribution of matrix proteins identified from cellular and decellularized fractions following sequential extraction with SDS and TX from FFPE and FR tissue. For this comparison the total number of matrix proteins identified in each fraction were compared and shown in Venn diagrams to illustrate overlap of protein identifications between extraction methods, tissue storage and cellular compartment:
4a. Distribution of matrix proteins following sequential extraction with SDS in FFPE tissue.
4b. Distribution of matrix proteins following sequential extraction with TX in FFPE tissue.
4c. Distribution of matrix proteins following sequential extraction with SDS in FR tissue.
4d. Distribution of matrix proteins following sequential extraction with TX in FR tissue.