Figure 4.

Pharmacological inhibition of glutathione reductase inhibits glutathione recycling in cystine-free medium. DMF still elevates cellular GSH when GSH synthesis is blocked by incubation in cystine-free medium. HT22 cells were treated for 24 h with 10 μM DMF (black bars) or vehicle (white bars) and then exposed to cystine-free medium for another 4 h in the presence of 50 μM BCNU (grey bars) before intracellular GSH was measured enzymatically. Graphs of all experiments represent the means ± SD of three independent experiments performed in triplicate. ^∗ p < 0.05, two-way ANOVA, and Tukey's post hoc test.