Table 3.

ASB3 gene mutations in colorectal cancer tissues and cell lines

Mutation genotype	Mutation type	Exon	Codon change	Amino acid change	Case ID/Cell line
Colorectal cancer tissues
CDS 21C→Ga	Nonsense	Exon 2	TAC→TAG b	Y7stop	C56
chr2:53810098 G→C	Splice-site	Exon 3/Intron	–	–	C53
CDS 404G→R	Missense	Exon 4	GGA→GAA	G135E	S78, S64
CDS 1084C→Y	Missense	Exon 8	CGC→TGC	R362C	S35
CDS 1016A→W	Missense	Exon 8	AAA→ATA	K339I	S12, S61, S64
Colorectal cancer cell lines
CDS 471A→T	Missense	Exon 5	GAA→GAT	E157D	HCT116
CDS 471A→W	Missense	Exon 5	GAA→GAT	E157D	HT-29
CDS 24G→R	Synonymous	Exon 2	GCG→GCA	Non	DLD-1
CDS 25G→K	Missense	Exon 2	GAC→TAC	D9Y	DLD-1
CDS 154T→Y	Missense	Exon 2	TAT→CAT	Y52H	DLD-1
CDS 922G→R	Missensee	Exon 7	GCC→ACC	A308T	DLD-1

ASB3, ankyrin repeat and suppressor of cytokine signaling (SOCS) box protein 3

The nucleotides in bold indicate the one mutated

^a CDS coding domain sequence; A, T, C, G, four types of nucleotides; R = A/G; Y = C/T; W = A/T; K = G/T

^bamino acid codon