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. 2017 Jan 14;17:14. doi: 10.1186/s12870-016-0958-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Detection of reactive oxygen species (ROS) in wheat after heat treatment at 45 °C for 1 h. a O₂ ⁻ accumulation in 7-day-old wheat leaves detected with NBT. b H₂O₂ accumulation in 7-day-old wheat leaves detected with DAB. c H₂O₂ content in 7-day-old JM5265 and transgenic wheat seedlings. d The activity of the antioxidant enzyme CAT in 7-day-old JM5265 and transgenic wheat seedlings. e The activity of the antioxidant enzyme GR in 7-day-old JM5265 and transgenic wheat seedlings. The data represent mean values ± SD of three independent experiments. (* indicates significance at P < 0.05; ** indicates significance at P < 0.01)