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. 2017 Jan 14;17:16. doi: 10.1186/s12866-017-0929-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Detection of B. microti presence in the qPCR positive patient samples by IFA (Top Panels) Two representative samples (J22 and J67) from Ocean and Monmouth Counties not tested for Babesia by microscopy at JSUMC show green fluorescence due to reactivity of antibodies in patient plasma with the parasites followed by detection with Alexa 488 conjugated secondary antibodies, when observed by using FITC filter indicating positive IFA results (marked by arrows). (Bottom Panels) Blue fluorescence due to DAPI staining shows the parasites present (marked by arrows) in each field of view of the Nikon 80i fluorescence microscope at × 1000 magnification when Apo-Plan TIRF objective was used. Scales shown represent respective panels of Giemsa-stained microscopy and IFA