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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Dec 10;482(4):1437–1442. doi: 10.1016/j.bbrc.2016.12.054

Figure 2. In vitro assembly of HIV-1 GagΔp6BH10 protein.

Figure 2

(A) GagΔp6BH10 formed large heterogeneous particles of diameter around 1–5 µm, when 1µg yeast tRNA was added to 5 µg GagΔp6BH10 diluted with buffer F. Inset: GagΔp6BH10 solution became turbid upon addition of yeast tRNA (right), while it remained clear in absence of yeast tRNA (left); (B–C) GagΔp6BH10 formed similar particles with addition of 20mer-Cy3 compared to yeast tRNA, determined by microscope bright field figure (B) and fluorescence image (C). (D) Co-localization of the large particles in bright field with the red fluorescent particles in fluorescence image when overlapping two images of the same field from Panel C and D. All figures are taken under light microscope with a magnification of 400 times, and the scale bar indicates 10 µm.