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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Dec 10;482(4):1437–1442. doi: 10.1016/j.bbrc.2016.12.054

Fig. 3. Microscopy photos and flow cytometry of in vitro assembly and disassembly of GagΔp6BH10 protein.

Fig. 3

(A) 5 µg GagΔp6BH10 was diluted with buffer F, and then mixed with 20mer-Cy3 at a molar ratio of nucleic acids to protein as 1 to 2. (B) After the assembly of GagΔp6BH10 with 20mer-Cy3 under the same conditions as in panel A, (dT)30 was added to the sample to reach a molar ratio of nucleic acids to protein as 8:1. After incubation at 21°C for 10 min, it was sampled and observed under microscope in fluorescence mode. The yellow scale bars indicate 10 µm for both panels. (C) Flow cytometry of the assembly formed by GagΔp6BH10 incubated with 20mer-Cy3. The sample was prepared under same assembly conditions as in (A), and analyzed 5 min after the addition of nucleic acids. (D) Flow cytometry of the assembly formed by GagΔp6BH10 first incubated with 20mer-Cy3 and then mixed with excessive (dT)30. The sample was prepared under same assembly conditions as in (B). After addition of excess (dT)30, the sample was incubated at 21°C for 10 min before flow cytometry analysis.