Figure 6.

Asiatic acid inhibits tumor growth in vivo. 1×10⁶ LLC cells were transplanted subcutaneously into the armpit of the C57 mice. Three days after transplantation, mice were randomly allocated to either control or treatment groups (n=6). Drugs were administered i.g. on days 1–13. (A) and (B) Tumor volume and mouse bodyweight were recorded daily. After sacrifice, solid tumors were separated and weighed. (C) and (D) The spleen index was calculated. (E) Paraffin sections of tumor tissues from mice were analyzed by H&E staining and TUNEL staining and (F) optical density was calculated. Scale bar, 50 μm. Data represent mean±SEM, n=8, ^*P<0.05, ^**P<0.01 vs. control.