Figure 5.

Detection of swelling in energized hepatic mitochondria (1 mg protein/mL). (A) In the presence of the various MitoCaA concentrations. (B) In the presence of 10 μmol/L MitoHCAs alone or 10 μmol/L MitoHCAs+Ca²⁺. (C) Inhibition of 20 μmol/L MitoCaA-induced mitochondrial swelling by 1 μmol/L of CsA. CsA was added as indicated by the arrows. The assays were performed ≥4 times with similar results. The values represent mean±SEM of three independent experiments. ^***P<0.001 vs. the control groups.