Fig. 5.
T-BHPH induced endothelial necroptosis Endothelial cells were incubated with t-BHP (0–500 μM) for 1 h, the cell viability, morphological alterations, LDH release, and the PI uptake were detected by MTT assay (A), microscopy (B), LDH assay kit (C), and PI staining (D), respectively. Cell were treated with t-BHPH for 1 h with Nec-1 (1 μM) and NSA (1 μM) alone or co-treatment for 1 h. The cell viability was examined by LDH assay kit (E). Results were expressed as mean±SD of three independent experiments. Nec-1, necrostatin-1; NSA, necrosulfonamide.