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. 2017 Jan 16;7:40256. doi: 10.1038/srep40256

Figure 5. Tight junctions and cell adhesion were affected by BACE1.

Figure 5

(A) BACE1 inh. (1 μM) was administered as pretreatment for 24 h, and then the cells were stimulated with TNF-α (50 ng/mL) for 24 h. EA.hy926 cell tight junctions were detected by transmission electron microscopy (10000 × magnification). (B) EA.hy926 cell tight junctions were also detected by confocal immunofluorescence analysis using anti-VE-cadherin antibody (Red). Blue, DAPI. (C) After BACE1 inh. (1 μM) treatment, cell adhesion assay was performed in EA.hy926 cells (100 × magnification). The data are the mean ± SD of three independent assays (**p < 0.01). (D) Transmission electron microscopy analysis was used to identify BACE1-overexpressing EA.hy926 cell tight junctions (10000 × magnification). (E) Immunofluorescent analysis of the tight junction protein VE-cadherin. Red, VE-cadherin. Blue, DAPI. (F) Monocyte adhesion to endothelial cells was quantified by monocyte adhesion assay. Dil-labeled THP-1 cells were incubated with endothelial cells in 96-well plates for 1 h at 37 °C. The plates were then washed three times with PBS, and the fluorescence was measured. The data are the mean ± SD of three independent assays (**p < 0.01).