Figure 5. Phylogenetic analysis of the transsulphuration pathway across parasitic protozoa.

(a) Phylogenetic tree was constructed using the Maximum Likelihood Method algorithm using sequences of the evolutionarily conserved Hsp90. The tree was drawn to scale and branch lengths correspond to number of substitutions per site. (b) To examine the evolution of the TS pathway, BLASTp analysis was performed to inspect the presence or absence of CBS and CGL across 11 phylogenetic relatives of Plasmodium taking Saccharomyces cerevisiae sequences as the template. Table shows the presence (+) or absence (−) of these enzymes in protozoan parasites.