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. 2017 Jan 16;7:40468. doi: 10.1038/srep40468

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Copyright © 2017, The Author(s)

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The miRNA level of miR-145 or mRNA levels of α-SMA or COL-I were examined by qRT-PCR. (a) miR-145 was examined by quantitative RT-PCR (qPCR) in the process of HSCs’ spontaneously activation. (b) The mRNA levels of α-SMA and TGF-β1 in HSCs treated with TGF-β. (c) Induction of miR-145 expression in rat primary HSCs by TGF-β stimulation. All experiments were repeated thrice with triplicate samples in each experiment. The relative value of miR-145 to U6 (or target mRNA to β-actin mRNA) was set as 1 in the control or day 0 HSCs. Data are presented by mean ± standard deviation. *p < 0.05.