Table 1. List of genes that were targeted for qRT-PCR analysis.
| Target Gene (encoded respiratory protein/subunit) | Primer Sequence |
|---|---|
| fdhA (formate dehydrogenase)* | 5′-GGCTTAGTTCCTTCTGGGAAAT-3′ |
| 5′-GAGTGAAGGTTAATCCAGGTGTAG-3′ | |
| frdA (fumarate reductase)*^ | 5′-GCGGTAATTTGTGAAGGAACAG-3′ |
| 5′-GGCACAATAGGAGTTGGATGA-3′ | |
| napA (nitrate reductase)^ | 5′-GATGAGCCATCAGGCTGTTAT-3′ |
| 5′-CCAAAGGATTGGGTGCATTTC-3′ | |
| nrfA (nitrite reductase)^ | 5′-ATCCGCTCTCATTTGAGCTTTA-3′ |
| 5′-CGCGATGAGCTAGGTAATATGG-3′ | |
| torA (trimethylamine N-oxide reductase)^ | 5′-GCCAGTGGTGTAAGTGTCTAA-3′ |
| 5′-ATAGCTCCACGCCCAAATAC-3′ | |
| cioA (cyanide-insensitive oxidase)^ | 5′-GGTGTGTGGCTATAGGAAGTAATC-3′ |
| 5′-TCTTGCTGTGTCAGGGTTAAAG-3′ | |
| ccoN (cb-type oxidase)^ | 5′-AGCGCAGCCATAGTCATAAA-3′ |
| 5′-CACTTGAAGGGCCAATTCTTTC-3′ | |
| cytC-1 (cyt c peroxidase)^ | 5′-CGCCCTAAATCTTCTTGGTTTG-3′ |
| 5′-TGGCTGCATCTCTTGTCATC-3′ | |
| cytC-2 (cyt c peroxidase)^ | 5′-TGGACAGCAAATCCTCATCAT-3′ |
| 5′-TCAGCTTGGATTGGACCTTTAG-3′ | |
| CJJ81176_0403 (putative oxidoreductase)^ | 5′-CCTACTGCAAGCAAGGTAAGA-3′ |
| 5′-CCCGATCAAGACGCTTTATTTC-3′ | |
| nrdA (ribonucleotide reductase) | 5′-CCTCAAGAATGGGCGAAGAA-3′ |
| 5′-GCGCGTTGTTCTTGCATTAG-3′ |
The primers were designed using the PrimerQuest Tool (Integrated DNA Technologies). *Respiratory proteins that facilitate the transfer of electrons from electron donors. ^Respiratory proteins that facilitate the transfer of electrons to electron acceptors. This list includes the majority of previously described respiratory acceptor proteins in C. jejuni12,18.