Figure 4. Demonstration of randomization restriction in a single whole plot and arrangement of whole plot, sub plot and sub-sub plot in split-split plot design in a full factorial design.

There are two randomization restrictions, photoperiod and temperature (HTC factors) hence the design is Split-Split Plot Design (SSPD). One level of photoperiod (whole plot) is selected and three levels of temperature (sub plot) was randomly allotted within the whole plot. Salinity is the sub-sub-plot factor which is easy to change (ETC). For this, within each of the three levels of temperature sub plot, three levels of salinity were randomly allotted.