Skip to main content
. 2017 Jan 16;7:40341. doi: 10.1038/srep40341

Figure 3. Measurements of the mean fluorescence intensity with a phase-sensitive flow cytometer.

Figure 3

Cytometric dot plots (n = 5000 cells) include (A) EGFP-E. coli cells counted without macrophage cells, (B) non-infected RAW264.7 cells, and (C) through (H) displaying RAW264.7 cell counts infected by EGFP-E. coli at times 5, 30, 60, 90, 120 and 180 min, respectively. Within Gate 1 from (A) through (H) mean fluorescence intensities are 9085 (CV = 3.33%; n = 4774), N/A, 8938 (CV = 3.03%; n = 373), 9062 (CV = 4.77%; n = 1236), 9494 (CV = 7.8%; n = 1861), 9691 (CV = 8.33%; n = 2096), 9704 (CV = 9.73%; n = 2866) and 9585 (CV = 9.84%; n = 3784), respectively; and mean SSC intensities are 8395 (CV = 1.19%), N/A, 8386 (CV = 1.22%), 8351 (CV = 1.06%), 8365 (CV = 1.22%), 8362 (CV = 1.12%), 8360 (CV = 1.14%) and 8341 (CV = 1.0%), respectively. Within Gate 2 from (A) through (H) mean fluorescence intensities are N/A, N/A, 9082 (CV = 4.57%; n = 4161), 11802 (CV = 12.84%; n = 3510), 15145 (CV = 10.68%; n = 3096), 15630 (CV = 9.28%; n = 2892)), 15894 (CV = 7.8%; n = 2102) and 15910 (CV = 7.69%; n = 1164), respectively; and and mean SSC intensities are N/A, N/A, 10361 (CV = 7.93%), 10372 (CV = 6.99%), 10628 (CV = 7.31%), 10722 (CV = 7.88%), 10887 (CV = 8.79%) and 10737 (CV = 9.15%), respectively.