Table 1. Model input parameters (V max and K m ) for L-leucine and L-phenylalanine transporters.
| Symporters | Antiporters | Uniporters | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SLC no. | SLC6A14 | SLC6A19 | SLC7A5 | SLC7A8 | SLC7A7 | SLC7A6 | SLC1A5 | SLC16A10 | SLC43A1 |
SLC43A2 |
||
| Alias | ATB0,+ | B0 AT1 | LAT1 | LAT2 | y+LAT1 | y+LAT2 | ASCT2 | TAT1 | LAT3 |
LAT4 |
||
| Accessory protein | coll TMEM27 | 4F2hc SLC3A2 | 4F2hc SLC3A2 | 4F2hc SLC3A2 | 4F2hc SLC3A2 | |||||||
| Vmax (nmol/h) | ||||||||||||
| Vmax1 | Vmax2 | Vmax1 | Vmax2 | |||||||||
| Leu | 0.014a46 | 0.53920 | 0.16247 | 0.40248 | 0.3849 | 1.6050 | 1.0151 | NA | 0.013817 | 0.76617 | 0.020418 | 3.51618 |
| Phe | 0.034a46 | 1.11820 | 0.14747 | 0.2748 | NA | NA | NA | 10027 | 0.007617 | 0.7517 | 0.020418 | 3.51618 |
| Km(mM) | ||||||||||||
| Km1 | Km2 | Km1 | Km2 | |||||||||
| Leu | 0.01252 | 1.153 | 0.03254 | 0.04855 | 0.031749 | 0.23650 | 0.36751 | NA | 0.084217 | 1.02417 | 0.10318 | 3.73318 |
| Phe | 0.01752 | 4.753 | 0.7454 | 0.012255 | NA | NA | NA | 3627 | 0.065817 | 1.20617 | 0.17818 | 4.69418 |
| Ala | 0.09952 | 4.153 | NA | 0.16755 | NA | 4.1250 | 0.018451 | NA | NA | NA | NA | NA |
| Arg | 0.10452 | NA | NA | NA | 0.3449 | 0.17750 | NA | NA | NA | NA | NA | NA |
| Trp | 0.02652 | >1253 | 0.021447 | 0.057648 | NA | NA | NA | NA | NA | NA | NA | NA |
| BCH | 0.52a52 | NA | 0.05556 | 0.02356 | NA | NA | NA | NA | 0.055b56 | 2.5ab17 | 0.055b56 | 28ab17 |
| Val | 0.03652 | 1.53a20 | 0.047247 | 0.12448 | NA | NA | 0.52251 | NA | 0.030617 | 1.88517 | 0.0472b56 | 36ab17 |
| Sodium dependent | yes | yes | no | no | yes | yes | yes | no | no | no | ||
| Log 10 mRNA | 4 | 0.6 | 1 | 2.5 | 3.4 | 3 | 3.5 | 0.8 | 2.4 | 1.5 | ||
References for model input are given as superscripts. As indicated all Vmax rates were reported as nmoles per hour (nmol/h). If the Vmax rates were not provided in units of nmol/h then these values were recalculated from literature reported units. aVmax and Km values were calculated using the Michaelis-Menten equation. bThe high affinity component was assumed to be equal to LAT1. NA (not applicable) indicates the amino acid was not reported to be a substrate for the transporter. The Log10 mRNA expression is taken from the reported microarray data for unfertilized X. laevis oocytes (Xenbase). The mRNA expression Log10 values for the accessory proteins, TMEM27/collectrin and SLC3A2/4F2hc, are 1.5 and 1.0 respectively. Standard three letter abbreviations are used for amino acid names; BCH is the Leu analog b(−)2-aminobicyclo[2,2, 1]heptane-2-carbocyclic acid.