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. 2017 Jan 16;7:40481. doi: 10.1038/srep40481

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The Rsc cells were harvested after exposure to VOCs from B. amyloliquefacians FZB 42 and B. artrophaeus LSSC22, benzaldehyde, 1,2-benz isothiazol-3(2 H)-one and 1,3-butadiene (1,3-BDN) for 72 hours. Real-time PCR was performed using a SYBR Green/Fluorescent qPCR master mix (Takara) on a Roche-480 system (Roche). The 16srRNA was used as an internal reference, and the transcriptional levels of PhcA, T3SS, T4SS pili, EPS and chemotaxis-related genes were detected. Each sample was replicated thrice for qPCR, and 2−ΔΔCt method was used to analyze gene expression level. Error bars indicate standard deviations of the means of three replicates.