Images illustrating CLSM in vivo detection of nitric oxide (NO) and peroxynitrite (green), peroxisomes (red) and chloroplasts (blue) in guard cells of transgenic Arabidopsis seedlings expressing CFP-PTS1. A and E, fluorescence punctates (red) attributable to CFP-PTS1, indicating the localization of peroxisomes in guard cells. B and F, fluorescence punctates (green) attributable to the detection in the same guard cells of nitric oxide and peroxynitrite, respectively. C and G, chlorophyll autofluorescence (blue) attributable to the detection of chloroplasts. D and H, merged images for corresponding panels.