Figure 5.

Effect of treatment on size of renal tumor cells in Tsc2^+/− mice.

Kidney sections were prepared from 13-month-old Tsc2^+/− mice after treatment and HE stained for estimation of average size of tumor cells. (A) Tumor (T) and adjacent normal (N) cells. Representative sections were presented to show size of tumor cells after 2-month treatment with vehicle, everolimus, sorafenib, and everolimus plus sorafenib. Scale bars are 100.1 μm. (B) Average size of tumor cells. Twenty-five lesions randomly chosen from each group after 2-month treatment were used to estimate average size of tumor cells using ImageJ. Everolimus alone or everolimus plus sorafenib significantly reduced average size of tumor cells. Sorafenib alone significantly reduced average size of tumor cells but to a smaller extent.