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. 2016 Nov 28;8(1):90–95. doi: 10.1021/acsmedchemlett.6b00392

Figure 1.

Figure 1

Induction of basal autophagy. ARPE-19 cells were treated for 4 h with DMSO, 23, or 22 without starvation. (A) Fold increase of LC3-II/actin ratios normalized to the DMSO control (n = 3). (B) Representative Western blot of LC-3 after treatment with 23 or 22 (25 μM). (C) Representative Western blot of LC-3 after treatment with 23 or 22 (50 μM). (D) Representative immunofluorescence detection of LC3 punctae after treatment with 23 or 22 (25 μM). Scale bar is 10 μm. CD63 was used as a late endosomal/lysosomal marker.