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. 2017 Jan 16;7:40719. doi: 10.1038/srep40719

Figure 2. ERAD is regulated by R1881 in a dose- and time-dependent manner.

Figure 2

(A) Androgen-starved LNCaP cells were treated with R1881 at indicated doses for 24 hour and the levels of ERAD components; AR and PSA were analyzed by immunoblotting using antibodies raised against them. (B) Androgen-starved LNCaP cells were treated with 10 nM R1881 for indicated times and expression levels were analyzed as in 2A. (C) Androgen-starved LNCaP cells were treated with 10 nM R1881 and the mRNA levels of the indicated genes were investigated using quantitative PCR (QPCR). Controls were treated with vehicle and set to 1. Data represent the mean of two independent biological replicates in triplicates and error bars represent SE. p-values were calculated with respect to vehicle-treated cells by two-tailed equal variance Student’s t-test. (*p < 0.005, #p < 0.05) (D) Androgen-starved LNCaP cells were pretreated first with 1 μg/ml cycloheximide or actinomycin for 1 h followed by R1881. 24 h after R1881 treatment, cells were lysed and the expression levels of the proteins of interest were tested using immunoblotting as in 2A.