Figure 2. Cell autonomous and non-autonomous effects of muscle PGC-1α in vitro.
(A,B) mRNA expression of anti-inflammatory cytokines (A) and IL-12 (B) in C2C12 myotubes adenovirally overexpressing PGC-1α or GFP. (C,D) RAW macrophages were treated with conditioned medium of C2C12 myotubes overexpressing PGC-1α or GFP and expression levels of pro- (C) and anti-inflammatory (D) cytokines were determined. Values represent the mean of at least 3 independent experiments +SEM. ***P < 0.001; PGC-1α versus GFP. See also Figure S1.